Journal: The Journal of Neuroscience

Article Title: Δ FosB Regulates Wheel Running

doi: 10.1523/JNEUROSCI.22-18-08133.2002

Figure Lengend Snippet: Cellular specificity of ΔFosBinduction by wheel running. Representative photomicrographs of rat brain sections from eight individuals demonstrating colocalization of ΔFosB-IR (brown stained nuclei) and dynorphin mRNA (black grains) (a) or ΔFosB-IR and enkephalin mRNA in the nucleus accumbens core (b).

Article Snippet: Forty-eight mer DNA oligonucleotide probes specific for dynorphin (296–345) ( Douglass et al., 1989 ) and enkephalin (235–282) ( Zurawski et al., 1986 ) mRNAs were radioactively labeled with [α- 35 S]dATP (DuPont NEN) in their 3′ ends using terminal deoxynucleotidyl transferase (Invitrogen, San Diego, CA) to a specific activity of ∼1 × 10 9 cpm/mg.

Techniques: Staining

Journal: The Journal of Neuroscience

Article Title: Δ FosB Regulates Wheel Running

doi: 10.1523/JNEUROSCI.22-18-08133.2002

Figure Lengend Snippet: Δ FosB in dynorphin and enkephalin cells in nucleus accumbens

Article Snippet: Forty-eight mer DNA oligonucleotide probes specific for dynorphin (296–345) ( Douglass et al., 1989 ) and enkephalin (235–282) ( Zurawski et al., 1986 ) mRNAs were radioactively labeled with [α- 35 S]dATP (DuPont NEN) in their 3′ ends using terminal deoxynucleotidyl transferase (Invitrogen, San Diego, CA) to a specific activity of ∼1 × 10 9 cpm/mg.

Techniques: Control

Journal: The Journal of Neuroscience

Article Title: Δ FosB Regulates Wheel Running

doi: 10.1523/JNEUROSCI.22-18-08133.2002

Figure Lengend Snippet: Expression of ΔFosB in 11B mice. Brain sections were analyzed for ΔFosB-IR (brown-stained nuclei) followed by in situ hybridization for dynorphin mRNA (A) or enkephalin mRNA (B) (black grains). Note the preferential expression of ΔFosB-IR in the enkephalin-positive but not the dynorphin-positive cells. Of 214 ΔFosB-positive cells counted in three 11B mice, 73 ± 11% were also enkephalin positive, and 22 ± 6% were also dynorphin positive. No double-labeling was seen between ΔFosB and interneuron markers.

Article Snippet: Forty-eight mer DNA oligonucleotide probes specific for dynorphin (296–345) ( Douglass et al., 1989 ) and enkephalin (235–282) ( Zurawski et al., 1986 ) mRNAs were radioactively labeled with [α- 35 S]dATP (DuPont NEN) in their 3′ ends using terminal deoxynucleotidyl transferase (Invitrogen, San Diego, CA) to a specific activity of ∼1 × 10 9 cpm/mg.

Techniques: Expressing, Staining, In Situ Hybridization, Labeling

Journal: The Journal of Neuroscience

Article Title: Δ FosB Regulates Wheel Running

doi: 10.1523/JNEUROSCI.22-18-08133.2002

Figure Lengend Snippet: Effect of ΔFosB overexpression on wheel running behavior in bitransgenic mice. A, Bitransgenic mice drinking tap water have inducible overexpression of ΔFosB in striatal dynorphin neurons (water) and showed increased running (distance per day) for the first 3 weeks of access to running wheels. In contrast, genetically identical littermate controls with doxycycline in their drinking water that do not overexpress ΔFosB (dox) showed increased running for the first 2 weeks only. B, Another line of the bitransgenic strain of mice, called 11B, with inducible overexpression of ΔFosB primarily in striatal enkephalin neurons (water) showed dramatically less running during their weeks 2 and 3 compared with genetically identical littermates that do not overexpress ΔFosB (dox). # indicates an increase in running (distance per week) within a group. * indicates a difference in running between the ΔFosBoverexpressers (water) and controls (dox). Vertical lines indicate borders between weeks 1 and 2, as well as weeks 2 and 3. Horizontal lines with the # symbol describe statistical differences between weekly running within a group. Data are expressed as mean (11A dox,n = 8; 11A water, n = 7; 11B dox, n = 6; 11B water, n = 7).#p < 0.05;##p < 0.01;###p < 0.001; *p< 0.05.

Article Snippet: Forty-eight mer DNA oligonucleotide probes specific for dynorphin (296–345) ( Douglass et al., 1989 ) and enkephalin (235–282) ( Zurawski et al., 1986 ) mRNAs were radioactively labeled with [α- 35 S]dATP (DuPont NEN) in their 3′ ends using terminal deoxynucleotidyl transferase (Invitrogen, San Diego, CA) to a specific activity of ∼1 × 10 9 cpm/mg.

Techniques: Over Expression